|
KMID : 0861020040190010089
|
|
Korea Journal of Herbology
2004 Volume.19 No. 1 p.89 ~ p.94
|
|
|
The Cytotoxic Effect of Benzoic Acid on Normal Human Skin Fibroblast
|
|
|
|
|
Abstract
|
|
|
Objective : The aim of this study was to evaluate cytotoxic effect of benzoic acid on normal human skin fibroblast cell line , Detroit 551.
Methods : The cytotoxicity was determined by colorimetric assays such as MTT assay or XTT assay. The microscopy was carried out to observe Detroit 551 cell line. Cells were incubated with various concentrations of benzoic acid in CQ incubator controlled with 37 c for 48 hours.
Results : The cytotoxic effect of benzoic acid on Detroit 551 did not show a positive significance at 50 uM and 100 uM benzoic acid by MTT assay or X T T assay compared with control. MTTB and XTTg were 900.6 uM and 974.6 uM, respectively. In microscopic study, Detroit 551 treated with benzoic acid showed a slightly decreased cell number, while the morphological changes of cell was not showed.
Conclusion : Above the results, the cytotoxicity of benzoic acid was midtoxicity on normal human skin fibroblasts cell line. Detrit 551
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|